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KMID : 1094720070120060618
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 6 p.618 ~ p.624
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Characterization of a novel β-hydroxy-β-methyl glutaryl coenzyme A reductase-inhibitor from the mushroom, Pholiota adiposa
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Yu Hyung-Eun
Lee Dae-Hyoung
Seo Geon-Sik
Cho Soo-Muk
Lee Jong-Soo
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Abstract
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This study describes the extraction and characterization of a novel inhibitor against β-hydroxy-β-methyl glutaryl (HMG) Coenzyme A (CoA) reductase from the mushroomPholiota adiposa. Methanol extracts ofP. adiposa PAD-022 fruiting body showed the highest HMG-CoA reductase-inhibitory activity of 55.8%. The HMG-CoA reductase-inhibitor,P. adipos a PAD-022, was maximally extracted when its fruiting body was treated with methanol at 30°C for 12 h. The HMG-CoA reductase-inhibitor was obtained by systematic solvent extraction methods followed by gel column chromatography, and RP-HPLC. The purified product was found to possess an activity of IC50 6.8 μg and a yield of 1.8%. The molecular weight of purified HMG-CoA reductase-inhibitor was deduced to be 412.7 Da. The inhibitor was identified as stigmasterol (C29H46O) by serial instrumental analyses, including LC-Mass. NMR, FT-IR, and UV spectrometry.
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KEYWORD
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β-hydroxy-β-methyl glutaryl coenzyme A reductase-inhibitor, Pholiota adiposaPAD-022, stigmasterol edible mushroom, methanol extracts
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